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Wikipedia
QIAGEN (, ) is a leading provider of sample and assay technologies for research in life sciences, applied testing, and molecular diagnostics. Founded in 1984, QIAGEN N.V. today is a Dutch holding company with operational headquarters in Hilden, Germa...
http://en.wikipedia.org/wiki/Qiagen
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Links
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Blogs
http://www.askstockguru.com/cgi-bin/s?su003dQGENu0026datu003d20080604When will Qiagen NV (down 1.26 percent, QGEN) see the light? (by: info@askStockGuru.com (AskS
Down -1.26% today. The stock has recovered 7.5% from its recent low price of 18.17 which occurred on 19-Mar-2008. A close above 20-day moving average of 21.1 could mark low of 18.17 as a recent low. The closest support can be found at ...
http://www.bioknow.cn/html/pages/461/Paper_53461.htmQIAGEN与上海Biochip 公司签订合作协议-- (by: unknown)
QIAGEN亚洲区将向中国首要药品开发及发展的供应商提供RNAi以及相应的解决方案Shanghai, May 30, 2008 - (ACN Newswire) - QIAGEN今天宣布已选定上海Biochip公司(SBC)作为首要合作伙伴，为SBC提供各种小干扰RNA (siRNA)库。SBC将使用此siRNA库建立中国首 ...
http://www.gbipharma.com/cpbheadlinefree.asp?newsidu003d2008561Qiagen to supply siRNA libraries to Shanghai Biochip --Free access (by: GBIpharma.com)
In its effort to establish China's first siRNA high-throughput screeni......
http://robperkin.blogspot.com/2008/05/qiagen-and-shanghai-biochip-corp-sign.htmlQIAGEN and Shanghai Biochip Corp. Sign Collaboration Agreement ... (by: Robin Perkin)
QIAGEN and Shanghai Biochip Corp. Sign Collaboration Agreement Japan Corporate News (press release), Japan - 1 hour ago Shanghai, May 30, 2008 - (ACN Newswire) - QIAGEN today announced that it has been selected by Shanghai Biochip ...
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Videos
joule.ram
Global Growth Companies Community Partner - Qiagen company
Peer Schatz CEO QIAGEN
Made in Germany | Qiagen -- Biotech Buy-Up
Qiagen: Nachweis von Vogelgrippe
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Downloads
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Definition
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Questions & Answers
In GTE miniprep solution I what is the glucose for?
In the standard miniprep protocol, the first solution is GTE (glucose-Tris-EDTA) and RNAse A. What is the purpose of the glucose, if you're going to lyse the bacteria anyway? I believe Qiagen buffer P1 does not have glucose and it works just fine.
What is the purpose of maintaining the osmotic balance if EDTA destabilizes the cell membrane anyway? Or are you saying that normally glucose is for maintaining the osmotic balance but in this case it acts as a nuclease inhibitor? As far as I have tried it, the glucose is dispensible for DH10B, DH5a, and derivative strains, or is it required for strains that have more nucleases?
Glucose is needed to keep the osmotic balance. You need that your bacteria do not break when you are resuspending them by vortexing. In this case glucose can help avoiding the destruction of DNA and RNA. I think you can avoid using it but you should always be gentle when adding sol2 (SDS/NaOH).
I think it can help not realeasing nucleases. I think it is a precaution, since you can find protocols which do not use glucose at all. I've used these methods and they work equally well.
QIAGEN Hybrid Capture II, QIAGEN Care HPV .Are these diagnstic tests for cervical cancer available i
QIAGEN Hybrid Capture II, QIAGEN Care HPV .Are these diagnstic tests for cervical cancer available in India?
So far as I know this facility is not available in India. Tata Memorial Hospital and AIIMS, New Delhi were to have them but I do not know the present status of availability. You can check up with them. -
What does AL. AW and AE stand for in the Qiagen DNA extraction?
@qaa_question
I'm not sure it stands for anything. Qiagen's buffers always have random letters and numbers to identify them. Instead of just being "resuspension buffer" it is "buffer L2".
Cause of low 260/230 ratio on Qiagen RNeasy kit for RNA extraction?
Ignore this, it's not a question. It's to help future people are googling this issue. I came across some yahoo answers a few times when I was trying to figure out the problem but no solutions work, so this is for anyone else in the future who has an issue.
First are the things we have heard on the internet and tried:
1. Heat up the RLT buffer to 37C before use. Let it cool a bit but once added to the frozen cell samples, take the samples off ice. The whole point is to stop the RLT from getting too cold and forming crystals.
2. Ensure your gloves aren't covered in white stuff (salty RLT) when you are doing the washes.
Now here is what solved the extremely annoying and expensive issue for us:
when doing the washes (RW1 and RPE) invert the column several times so that the buffers can wash the lid. Then, roll the spin column (like you would roll a barrel) with your fingers to make sure the buffer has really washed the inside of the tube.
This makes sense; the buffer volumes (700ul and 500ul) are not enough to fill the column and reach the lid. If you don't do this, you will notice small white crystals all over the underside of the cartridge lid. This is RLT, and it can come down during the elution. Also, rolling the wash makes it actually wash the cartridge, as opposed to just sitting there. It's possible that this is why you are supposed to squirt the RNAse free water directly onto the filter, but we have not tested this.
here's some keywords so people can find this:
RLT contamination
low 260/230 ratio
bad 260/230 ratio
contaminated RNA
guanidine contamination
Here's an example of everyone disagreeing on the issue:
http://www.protocol-online.org/biology-forums/posts/6412.html
A ratio of 1.8 or higher is needed for array processing.
Also, something else you might try is sucking the flow through out of the collection tubes with an aspirator instead of just dumping them out (as dumping them out isn't nearly as clean).
Qiagen also recommended letting the RPE and RW1 remain in the cartridge for about 2 minutes so that they can wash. (Doing the inversion and rolling of 16 samples as I recommend above easily takes 2 minutes anyway).
Good luck. Also, if using the qiagen tissue lyser, make sure you know that the farther out the samples are in the blocks, the more they get shaken and the more lysis occurs. (There are 3 rows, use the row farthest away from the lyser)
No offense but your post is pretty bad... Labs do not have the time or the money to spend getting bad RNA and finally figuring out its because they didnt invert the cartridge...that does not teach them anything about science, it only gets rid of lab money. There is nothing wrong with what I'm doing.
You are also arguing two different things, it's fine that you have a problem with the kits since they teach nothing about isolating RNA...but you should have no problem helping people skip little issues when it comes to using the kit. Telling people to invert and roll the cartridge has nothing special to do with scientific methods for the experiment...
I think most people who uses this kit already knows it ... or let them trouble shoot things .. trouble shooting is the way to learn issues. students in lab now a days have no idea why and how a certain experiment works because of kits like this .... giving them a chance to trouble shoot will give them an opportunity to actually look into the process and methods.
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